Antioxidant potential of alpha-tocopherol against flubendiamide and copper-induced alteration in redoxstate of erythrocytes

Present study was conducted to evaluate the antioxidant effect of α-tocopherol against flubendiamide and copper-induced alteration in oxidative stress in erythrocytes following 90 days concurrent administration. Wistar rats were divided into nine groups of six animals each. Saline (I), corn oil (II) and α-tocopherol (100 mg/kg) (III) groups were controls, while next three groups (IV, V and VI) were orally exposed with copper sulphate (33 mg/kg), flubendiamide (200 mg/kg) and copper sulphate + flubendiamide, respectively. VII, VIII and IX groups were concurrently treated with copper sulphate + α-tocopherol, flubendiamide + α-tocopherol and copper sulphate + flubendiamide + α-tocopherol, respectively for 90 days. Lipid-peroxidation (LPO) level was significantly higher (P<0.05) in copper and copper + flubendiamideexposed erythrocytes while superoxide dismutase (SOD) activity significant reduces (P<0.05) in copper and/or flubendiamide exposed groups. Glutathione-S-transferase (GST) activity was significantly (P<0.05) higher in copper and flubendiamide alone and combined exposed groups, while, glutathione-peroxidase activity (GPx) was significantly (P<0.05) lower in vehicle control, α-tocopherol and copper alone exposed rats. No significant alteration was observed in activity of Catalase and GSH and Total protein levels in any of the xenobiotics as well as control and treatment groups. Concurrent treatment of α-tocopherol with copper/flubendiamide showed decrease in LPO level and improvement in SOD, GST and GPx activities in RBCs comparable to the control groups indicate amelioration potential of α-tocopherol.