Morin potentiates duodenal motility in arsenic intoxicated swiss albino mice

The aim of the study was to study the effect of morin (a phytochemical) on duodenal motility in arsenic intoxicated mice. Swiss albino mice of 5–6 weeks of age were divided into three experimental groups containing six animals each such as; Gr-I (untreated and control), Gr-II (Arsenic trioxide @ 3 mg/kg bwt. p.o. for 28 days), Gr-III (morin @ 50 mg/kg bwt. p.o. + Arsenic trioxide @ 3 mg/kg bwt. p.o. for 28 days). Morin was administered 30 min before oral administration of arsenic trioxide. On 29th day the animals were sacrificed and a small piece of intestine from the duodenal part was collected in aerated chilled MKHS solution. 20 mm strips of the duodenum were mounted in organ bath containing 20 ml MKHS solution and given a resting tension of 0.5g and allowed one hour incubation period. After incubation period ACh (1nM-10uM) dose response curve was studied in viable rings. pD₂ of ACh in control was 6.95±0.18 which was decreased to 5.89±0.06 in arsenic intoxicated animals. This was elevated towards control value by administration of morin @50mg/kg (pD₂= 7.04±0.22). EC₅₀ in control (1.13×10⁻⁷M) which was increased to 1.31×10⁻⁶ M in arsenic intoxicated group. EC₅₀ was significantly decreased to 9.21×10⁻⁸M which was very significant. In conclusion, from the functional study it was observed that morin at 50 mg/kg orally has potential effect on increasing duodenal motility and function in arsenic intoxicated swiss albino mice.