Ameliorative effect of itk formulation in chromium pre- exposed diabetic rats

The objective of present study was to evaluate the influence of pre-exposure of chromium @ 1 mg/kg body weight by oral gavage continuously for 30 days in experimentally streptozotocin-induced type 2 diabetes in male Wistar rats and after 30 days of continuous exposure ameliorative potential of an ITK formulation @ 435 mg/kg body weight and its comparative efficacy with metformin @ 50 mg/kg body weight on diabetes-induced dysfunction. Thirty six male Wistar rats were divided into six groups viz. Normal Control, Diabetic, Chromium exposed, Chromium pre-exposed + Diabetes, Chromium pre-exposed + Diabetic + Metformin, Chromium pre-exposed + Diabetes + ITK, consisting six animals in each group, were induced diabetes with streptozotocin @ 30 mg/kg body weight, intraperitoneally single dose after 30 days of pre-exposure to chromium. After induction of diabetes ITK formulation and metformin were given by oral gavage continuously for 30 days. Diabetic rats and chromium pre-exposed diabetic rats increased feed and water intake and decrease in body weight, percent weight gain, anthropometric parameters, hemoglobin, TEC and platelets count where as significant increase in TLC. Chromium exposed group did not show any significant changes. Treatment with ITK formulation and metformin moderately to significantly improved feed and water intake and increase in body weight, percent weight gain and anthropometric parameters, hemoglobin, TEC and platelets count where as significant decrease in total leucocyte count. In conclusion, the protective effect of ITK and chromium against type II diabetes may be explored to counter the various defects produced in type II diabetes after conducting further detailed studies in future.