FTIR analysis for identification of functional compounds of leaf powder and ethanolic extract of Azadirachta indica A. juss

Azadirachta indica (neem) is an ascent medicinal plant found in Africa, America and other tropical regions. A large number of bioactive compounds are present in seed, leaf, flower, bark, and roots of neem, which guarantee great versatility in their use. Neem causes deleterious effects on the fertility of mammals and insects. The present study was carried out to identify the functional compounds through Fourier Transform Infrared Spectroscopy (FTIR) in dried leaf powder and ethanolic leaf extract of Azadirachta indica and its significance with male contraception. The vibration assignments, intensities and wave numbers of dominant peaks were obtained by absorption of the spectrum. Various functional groups like O-H, N-H, C-N, C=C, C-O, C=O were identified and representing flavonoid, steroid, alkaloid, tannins, terpenoids and saponins like biomolecules, which might be responsible for contraception in male by altering the steroidal hormones and hypo-thalamus-pituitary-testis axis.