Journal Of Veterinary Pharmacology And Toxicology

  • Year: 2021
  • Volume: 20
  • Issue: 2

Pharmacodynamic study of methanolic extract of seeds of Entada phaseoloides on cholinergic, histaminergic and serotonergic receptors using isolated tissue preparations

  • Author:
  • Pranay Talukdar1, Chandana Choudhury Barua1,*, Beenita Saikia1, Jadav Sarma1, Rumi S. Borah2, Shantanu Tamuly3
  • Total Page Count: 8
  • DOI:
  • Page Number: 76 to 83

1Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Guwahati -781022, Assam, India

2Department of Livestock Production and Management, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam-781022, India

3Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam-781022, India.

Abstract

The pharmacodynamic effect of methanolic extract of seeds of Entada phaseoloides (MEEP) on cholinergic, histaminergic and serotonergic receptors using isolated tissue of rat ileum, guinea pig ileum and rat fundus preparation are presented in this paper. The concentration response curve (CRC) of standard agonist Ach, histamine, 5-HT and the test drug MEEP (30 to 3000 mg/ml) and MEEP in presence of standard antagonist atropine (10“6 M), chlorpheniramine (10“5 M) and ketanserin (10“6 M) were recorded on rat ileum, guinea pig ileum and rat fundus respectively. Ach, histamine, 5HT and MEEP produced concentration-dependent contractions on isolated tissue of rat ileum, guineapig ileum, rat fundus which indicate agonistic activity on cholinergic, histaminergic and serotonergic receptor system. EC50 of ACh in presence of atropine was significantly higher (P < 0.001) than EC50 of ACh. Similarly, EC50 of MEEP in the presence of atropine was significantly higher (P < 0.001) than EC50 of MEEP. EC50 of histamine and MEEP in presence of chlorpheniramine was significantly higher (P < 0.001) than EC50 of histamine. Finally, EC50 of 5-HT in presence of ketanserin was significantly higher (P < 0.001) than EC50 of 5-HT. EC50 of MEEP in presence of ketanserin (10"6 M) was significantly higher (P < 0.001) than EC50 of MEEP. From this study, it may be inferred that MEEP possesses agonistic activity on cholinergic, histaminergic and serotonergic receptors. Consequently, seeds of Entada phaseoloides can be effectively used in gastric disorders like constipation, gastric emptying, diabetes, obesity and also in mood disorders.

Keywords

Entada phaseoloides, Fundus, Guineapig, Ileum, Ketanserin