Ameliorative effect of ITK formulation in arsenic pre-exposed diabetic rats

The goal of the present study was to assess the effects of pre-exposure to arsenic at a daily level of 38 ppb in drinking water for 30 days on experimentally inducing type 2 diabetes in male Wistar rats. Ameliorative capacity of an ITK formulation at a concentration of 435 mg/kg b wt and its comparative effectiveness with metformin at a concentration of 50 mg/kg b wt in diabetes-induced dysfunction was evaluated after 30 days of continuous exposure to arsenic in rats. Thirty-six male Wistar rats were divided into 6 groups, each with 6 animals: normal control, diabetic, arsenic exposed, arsenic pre-exposed + diabetes, arsenic pre-exposed + diabetic + metformin and arsenic pre-exposed + diabetes + ITK. After 30 days of pre-exposure to arsenic, diabetes was induced with single dose of streptozotocin at 30 mg/kg b wt, ip. Following the onset of diabetes, ITK formulation and metformin were given orally and consistently for 30 days. The feed and water intake of diabetic and arsenic exposed diabetic rats were increased and body weight, anthropometric measurements, hemoglobin, total erythrocyte count and platelet count decreased, while the total leucocyte count increased significantly. Arsenic exposed group did not exhibit any appreciable alterations. Treatment with metformin and ITK formulation moderately to significantly improved feed and water consumption, increased body weight and anthropometric measurements and increased hemoglobin, total erythrocyte and platelet counts, while significantly reducing total leucocyte count.In conclusion, the beneficial impact of ITK against arsenic pre-exposed type II diabetes may be explored to mitigate the multiple defects developed in type II diabetes after performing further extensive analysis in future.