Antimicrobial Effect of Tectona grandis Leaves against Bacteria Isolated from Canine Wound Samples

Thirty five canine samples were collected over a period of 90 days from Madras Veterinary College Hospital, Chennai, Tamil Nadu. Initial screening of Staphylococcus (57.14%) and E. coli (34.28%) was done by MSA and EMB agar respectively. Antibiogram of the organisms was done with the antibiotics including Amikacin (30mcg), Ceftriaxone (30mcg), Cepholothin (30mcg), ampicillin (10mcg), Gentamicin (10mcg), Cefotaxime (30mcg), Penicillin-G (10U), Tetracycline (30mcg), Ciprofloxacin (5mcg), Amoxycillin + clavulanic acid (30 mcg) by using Kirby-Bauer disc diffusion method. The various concentrations of methanolic teak leaf extract (20µl, 40µl, 60µl, 80µl) were used with amoxyclav to study the combined effect against the mixed wound culture. Among the used antibacterial agents in this study, the amoxycillin (90%) and ampicillin (84%) were showed highest bacterial resistance. Combined effect of amoxyclav and methanolic teak leaf extract (20µl, 40µl, 60µl, 80µl) showed dose dependent zone of inhibition against mixed wound culture. In conclusion we can use methanolic teak leaf extractalong with the amoxyclavto reverse the resistance of mixed bacterial culture from wound to the amoxyclav.