The study evaluated the pharmacokinetics and tissue residue levels of amoxicillin in broiler birds administered orally at 20 mg.kg-1 b.w., as a single dose. Plasma and tissue samples were collected and analyzed using HPLC. After a single dose, amoxicillin showed a distribution half-life (t1/2α) of 0.990 ± 0.171 hours, elimination half-life (t1/2β) of 3.249 ± 0.345 hours, a volume of distribution (V/F) of 5.671 ± 0.203 L.kg-1, and a maximum plasma concentration (Cmax) of 1.674 ± 0.082 μg.mL-1. Tissue residue analysis revealed the highest concentrations in the kidney (0.207 ± 0.027 μg.g-1) and liver (0.149 ± 0.016 μg.g-1) 24 hours after a single dose. Minimal residues were found in the heart, muscle, lungs, and spleen, while fat tissues showed no residues. No detectable residues were present in the spleen, heart, lungs, muscle, and fat after 72 hours. Based on these findings, a withdrawal period of three days is recommended for single-dose oral administration in broiler birds.
Pharmacokinetics, Single dose, HPLC, Broiler
