1Department of Veterinary Pharmacology and Toxicology, Bihar Veterinary College, Bihar Animal Sciences University, Patna - 800 014, Bihar, India
2Department of Veterinary Biochemistry, Bihar Veterinary College, Bihar Animal Sciences University, Patna - 800 014, Bihar, India
3Department of Veterinary Pathology, Bihar Veterinary College, Bihar Animal Sciences University, Patna - 800 014, Bihar, India
*Corresponding Author: E-mail: drnkvet@gmail.com
Online published on 23 July, 2025.
This present study was conducted to evaluate the In vitro antioxidant activity of two polyherbal formulations containing four medicinal plants viz. Trigonella foenum-graecum (Fenugreek), Momordica charantia (Bitter melon), Syzygium cumini (Jamun) and Catharanthus roseus. All these medicinal plants are well known for their antidiabetic properties. Since, diabetes and oxidative stress are positively correlated, hence, assessing the antioxidant status of the medicinal plants as well as polyherbal formulations can reflect about their antidiabetic capacity. These plants were tested using two antioxidant assays viz. DPPH and ABTS radical scavenging assays. The hydromethanolic extracts of all plants exhibited maximum antioxidant activity as compared with aqueous and methanolic extracts. Also, the hydromethanolic extracts exhibited maximum total phenol and flavonoid content. Hence, hydromethanolic extract of the polyherbal formulations were selected for further studies. Out of four plants, Syzygium cumini (SCHM) exhibited stronger antioxidant activity with IC50 values of 53.19 μg/mL (DPPH) and 74.81 μg/mL (ABTS), followed by Trigonella foenum-graecum (IC50 values of 80.86 μg/mL for DPPH) and 96.07 μg/mL for ABTS), Momordica charantia (IC50 values of 110.57 μg/mL for DPPH and 127.66 μg/mL for ABTS), while Catharanthus roseus showed least antioxidant property (IC50 values of 129.48 μg/mL for DPPH and 186.42 μg/mL for ABTS assays. The polyherbal formulations PHF-I and PHF-II demonstrated antioxidant activity with IC50 values of 75.78 μg/mL (DPPH) and 104.15 μg/mL (ABTS) for PHFIand IC50 values of 104.20 μg/mL (DPPH) and 127.93 μg/mL (ABTS) for PHF-II.The antioxidant values were compared with standard ascorbic acid (IC50 values of 3.95 μg/mL and 18.97 μg/mL for DPPH and ABTS assays, respectively). These results highlight significant antioxidant activity in the hydromethanolic extract of PHF-I, suggesting its superior therapeutic efficacy as compared to PHF-II.The findings aim to support their traditional usage and enhance understanding of their role in managing oxidative stress.
Catharanthus roseus, In vitro antioxidant activity, Momordica charantia, Polyherbal formulation, Syzygium cumini, Trigonella foenum-graecum
