The current study was carried out to explore the disposition kinetics and tissue residue study of cloxacillin in poultry birds following single dose (25 mg.kg-1b.w.) oral administration. The cloxacillin concentration in plasma and tissue was analyzed by HPLC (High Performance Liquid Chromatography. The pharmacokinetic parameters calculated were Cmax (0.453 ± 0.009 μg.mL-1), Tmax (0.322 ± 0.007 h), elimination half-life (t1/22) (11.412 ± 0.668 h), distribution half-life (t1/2±) (0.202 ± 0.006 h), mean area under the curve (AUC) (4.103 ± 0.566 μg.mL-1.h), clearance (CL/F) (6.844 ± 1.128 L.Kg-1. h-1) and volume of distribution (V/F) (23.588±0.552 L.kg-1). The highest mean residue concentration of cloxacillin following single oral dose (25 mg.kg{ 1 b.w.) administration was detected in liver 0.213 ± 0.011 μg.g-1 and the lowest in the lungs 0.106±0.0006 μg.g-1at 24 h. While no residues were detected in heart and fat tissue at 24 h. After 48 hours and 72 hours of oral administration, no drug residues were detected in liver, kidney, muscle, lung, spleen, fat, and heart. In this study, the initial dose of cloxacillin was 28.18 mg.kg{ 1, followed by a maintenance dose of 8.54 mg.kg{ 1 every 4 hours. A 2-day withdrawal period is recommended after administering a single oral dose of 25 mg.kg{ 1 to poultry birds.
Disposition kinetics, Single dose, HPLC, Poultry
