Assessment of antiurolithic activity of aqueous extracts of Boerhavia diffusa in wistar rats

In the present study antiurolithic activity of aqueous extract of Boerhavia diffusa was investigated in wistar rats. Urolithiasis was induced in ratls by using ethylene glycol (0.75 % v/v) in drinking water for 28 days. The aqueous extracts of Boerhavia diffusa was administered @ 100, 200 and 400 mg/kg body weight orally for 28 days along with 0.75% ethylene glycol in treatment group, respectively. Anti-urolithiatic activity of aqueous extracts of Boerhavia diffusa were assessed by studying haematological parameters, serum biochemical parameters and histopathological examination of tissues. Hematological estimation like RBCs, haemoglobin, packed cell volume, MCV, MCH, MCHC, WBCs, lymphocyte %, neutrophil % and platelets counts was carried out. Serum biochemical parameters like alanine amino transferase, aspartate amino transferase, alkaline phosphatase, urea, blood urea nitrogen, creatinine, uric acid, calcium, phosphorus and magnesium were quantified by using standard assay kits. Results revealed that the mean value of urea, BUN, creatinine, uric acid, calcium and phosphorus significantly decreased in rats treated with 200 and 400 mg/kg aqueous extracts of Boerhavia diffusa as compared to control group. Result of the present study indicated that aqueous extracts of Boerhavia diffusa possesses significant antiurolithic activity in rats.