Evaluation of flubendiamide toxicity and its amelioration with curcumin in wistar rats

Flubendiamide, considered as safest phthalic acid diamide insecticidewas used in the present study to evaluate sub-acute toxicity in male Wistar rats and its possible amelioration with curcumin. The toxicity and its amelioration were considered on the basis of feed and water intake, weekly body weight and percentage weight gain, absolute and relative organ weight, hemato-biochemical profiles and oxidative stress indices in erythrocytes Forty-two Wistar rats of either sex were divided into seven groups viz. group I-III includes control, vehicle control and curcumin (100 mg/kg body wt.), group IV-VI received flubendiamide @ 100, 200 and 300 pmm, respectively while group VII (flubendiamide @ 200ppm and curcumin @ 100 mg/kg body weight).Flubendiamide @ 200 and 400 ppm significantly reduced feed intake and water intake @ 400 ppm only but no significant change in body weight and percentage weight gain appreciable and significant change was not recorded in absolute and relative organ weights. Dose-dependent decrease in hemoglobin and TEC level and increase in platelet count, eosinophils and neutrophils at 400 ppm of flubendiamide were observed. Flubendiamide @ 400 ppm also increased total protein and cholesterol however non-significant alterations in ALT and AST activity, and an increase in lipid peroxidation and GST activity at 400 ppm. Curcumin in combination with flubendiamide has been observed to restore the values of different general, hemato-biochemical and oxidative stress indices towards control values and improved the health of the animals.