Legume Research - An International Journal
Web of Science
  • Year: 2001
  • Volume: 24
  • Issue: 3

Electrophoretic studies on seed protein profile of mungbean

  • Author:
  • B.S. Naik, C. Kole
  • Total Page Count: 5
  • Page Number: 169 to 173

Laboratory of Molecular Biology and Biotechnology, Orrisa University of Agriculture and Technology, Bhubaneswar - 751 003, India

Online published on 29 October, 2012.

Abstract

SDS-polyacrylamide gel electrophoresis of total seed proteins of 37 mungbean genotypes evidenced for the presence of 23 polypeptide bands dispersed over five zones, A to E within a molecular weight range of 17.4 to 75.0 kD. The banding patterns exhibited polymorphism for the presence of a band of 62.4 kD, B1 or two bands present jointly (61.0 and 58.2 kD), B2+ B3 indicating absence of gene(s) involved in post-translational dissociation in the genotypes without these two bands. Another band of 30.2 kD, D2(D2α) had a high molecular weight variant (D2β) of 31.3 kD. This is the first report on a detailed electrophoretic banding pattern of mungbean based on a large number of Indian cultivars.