Analysis of variability in different genotypes of pea (Pisum sativum L.) on the basis of protein markers

Seeds of 10 varieties of pea procured from Punjab Agricultural University, Ludhiana and Haryana Agricultural University, Hissar, India and grown in research farm at Panjab University, Chandigarh for three successive generations were analyzed for total protein and banding patterns of protein fractions. Significant varietal variations in the mature dry seeds of pea genotypes for total protein (14.95% to 22.44%), albumins (3.30% to 6.35%), globulins (7.97% to 10.53 %), glutelins (1.15% to 3.05 %) and prolamins (0.46% to 1.50 %) were observed for the means of three years. Seed storage protein banding profile was also studied by performing SDS-Polyacrylamide gel electrophoresis. A total of 20-21 bands were observed in all the ten genotypes. Except for some minor differences in the intensity of the bands, major differences were not recorded in the protein profiles.