Sodium Azide-Induced Mutagenesis in Fenugreek (Trigonella Foenum Graecum Linn)
Abstract
Fenugreek (Trigonella foenum graecum L.), a leguminous annual plant belonging to the family Fabaceae. Pre-soaked seeds were treated with different concentrations- 1.5 mM, 3.0 mM, and 4.5 mM of sodium azide for 4, 6, and 8 hours duration each. The mutagen treatments shifted the mean values of traits in positive as well as negative direction as compared to control. Number of branches reduced with the increasing doses of sodium azide. Considerable increase in variance was recorded for all the traits studied. The present results revealed that the induced variation with 4.5 mM sodium azide applied for 4 hours could be recommended for the selection of new genotypes of fenugreek with improved seed yield.
Keywords
Chemical mutagens, Sodium azide, Fenugreek, Genetic variability