Legume Research - An International Journal
Web of Science
  • Year: 2011
  • Volume: 34
  • Issue: 2

Development Of Rapd/Scar Marker for Yellow Mosaic Disease Resistance in Blackgram

  • Author:
  • L. Prasanthi, B.V. Bhaskara Reddy, K. Rekha Rani, R. Mehala Devi, B. Geetha, Y. Siva Prasad, K.Raja Reddy
  • Total Page Count: 5
  • Page Number: 129 to 133

Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati-517 502, India.

Abstract

A total of 130 random oligonucleotide primers were surveyed to identify yellow mosaic resistant genotypes in blackgram. Primer OPQ-1525 was found to produce a consistent marker, which differentiated resistant genotype from susceptible genotype. An identified random amplified polymorphic DNA (RAPD) marker, OPQ-1, linked to YMV resistant was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region primer SCAR (20f/r). The marker designed was amplified at a specific site of 532bp only in resistant genotypes. This would help for screening different genotypes and segregating generations in marker-assisted breeding programme.

Keywords

Blackgram, YMV, RAPD, SCAR marker