Legume Research - An International Journal
Web of Science
  • Year: 2013
  • Volume: 36
  • Issue: 1

Evaluation of antioxidant activity, cytotoxic studies and GC-MS profiling of matthiola incana (stock flower)

  • Author:
  • Nasir Rasool1,, Sania Afzal1, Muhammad Riaz1, Umer Rashid2,, Komal Rizwan1, Muhammad Zubair1, Shafqat Ali3, Muhammad Shahid
  • Total Page Count: 12
  • Page Number: 21 to 32

1Institute of Advanced Technology, Universiti Putra Malaysia, Serdang-43400, Selangor, Malaysia

2Department of Chemistry, Quaid-i-Azam University, Islamabad-45320, Pakistan

3Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan

Department of Chemistry, Government College University, Faisalabad-384 00, Pakistan

*Correspondig Author's e-mail: nasirhej@yahoo.co.uk; umer.rashid@yahoo.com

Online published on 28 March, 2013.

Abstract

Antioxidant activity of Matthiola incana flowers extracted with different solvents like petrolewn ether, chloroform, ethyl acetate, absolute methanol, methanol water (9.5:0.5) methanol water (9:1) extracts of stock flower (Matthiola incana) were analyzed. The total phenolics content (TPC) were fOWld in the range of 0.321–1.812 mg GAF/g and total f1avonoids content (TFC) were found in the range 2.06–6.061 CEmg/g. Other parameters studied were reducing power, DPP Hradical scavenging and inhibition of linoleic add per oxidation. The antioxidant activity of the Matthiola incana was also evaluated by measuring the conjugated dienes (CD), conjugated triene (CT), p-anisidine value (PAV), free fatty add (FFA) and peroxide values (PV) by using canola oil as an oxidative substrate. The n-hexhane extract was also analyzed by GC-MS. The methanolic extracts of Matthiola incana flowers showed maximum antioxidant activity. The cytotoxidty studies was also studied by haemolytic activity of the plant extracts against the hwnan erythrocytes (RBC's) was found to be in the range of 0.91–4.41%.

Keywords

Antioxidants, Matthiola incana, Phenolics, DPPH, cytotoxicity studies