In-silico validation and comparative analysis of candidate gene encoding proline rich protein in Lens culinaris

In present study a proline rich protein has been identified in lentil EST database using (soyabean) glycine max proline rich protein sequence as query sequence known to be down regulated in response to salinity and drought stress. Sequence similarity search has shown 90% similarity with chickpea EST sequence Accesion no. GR 398344 and 85% similarity with Lens culinaris EST sequence Accession no. GT 622346. Primer pairswere designed for thissequence and validated on selective Lens culinaris cultivars. tblastx result has also shown similarity with the glycine max (Soybean) proline rich protein showing repetitive pattern of amino acids. Repetitive pattern observed was similar to the characteristic pattern present in proline rich protein. Identification of this gene will further help to study the role of proline rich protein in response to various biotic and abiotic stress.