Legume Research - An International Journal
Web of Science
  • Year: 2015
  • Volume: 38
  • Issue: 1

Identification of AFLP markers linked to Fusarium wilt disease in pigeonpea [Cajanus cajan (L.) Millsp.]

  • Author:
  • B.C. Ajay, M. Byre Gowda, P.S. Prasad, G.N. Veerakumar4, H. Prashanth Babu, B.N. Gnanesh1, S.C Venkatesh3, K.N. Ganapathy2, R. Abdul Fiyaz, K.T. Ramya4
  • Total Page Count: 5
  • Page Number: 126 to 130

1ICRISAT, Patancheru, Hyderabad

2NRC for Sorghum, Hyderabad

3Dept. of Crop Improvement & Biotechnology, University of Horticultural Sciences, College of Horticulture, Mysore

4ICAR Research Complex for North Eastern Hill Region, Meghalaya, India-793103

AICRP on Pigeonpea, ZARS, Uniersity of Agricultural Science, GKVK, Bengaluru

*Corresponding author: ajaygpb@yahoo.co.in

Online published on 25 February, 2015.

Abstract

An experiment was conducted to identify markers linked to Fusarium wilt disease resistance, Parents namely TTB 7 and ICP 8863 were screened using 151 SSRs markers and 16 AFLP primer combinations. Parental screening revealed five SSR primers and 12 AFLP primer combinations polymorphic between parents. Bulk segregant analysis identified five AFLP primer combinations generating seven markers polymorphic between resistant and susceptible bulks while, none of the SSR markers were polymorphic. This indicates that, these markers are putatively linked to wilt disease. Screening of F2 segregating population of cross TTB 7 x ICP 8863 with these putatively linked markers revealed four markers (E-AAT/M-CTG850, ETCG/M-CTT650, E-TCG/M-CTA730 and E-TCG/M-CTT230) which segregated in 3:1 mendelian pattern. Simple linear regression performed on these four markers had identified two markers namely E-TCG/M-CTT650 and E-TCG/M-CTA730 linked to disease.

Keywords

AFLP, BSA, Fusarium wilt disease, Linkage, Pigeonpea