Legume Research - An International Journal
Web of Science
  • Year: 2017
  • Volume: 40
  • Issue: 1

Regeneration of plantlets from immature explants culture in Glycine max (L.) Merrill

  • Author:
  • Nidhi Pathak1,, Sharad Tiwari2, Manish Kumar Mishra1
  • Total Page Count: 5
  • Page Number: 69 to 73

1Department of Plant Breeding and Genetics, Jawahar Lal Nehru Krishi Vishwa Vidhyalaya, Jabalpur, Madhya Pradesh, 482004

2Biotechnology centre, Jawahar Lal Nehru Krishi Vishwa Vidhyalaya, Jabalpur, Madhya Pradesh, 482004

Department of Plant Breeding and Genetics, Jawahar Lal Nehru Krishi Vishwa Vidhyalaya, Jabalpur-482 004, Madhya Pradesh, India

*Corresponding author's e-mail: nidhijnkvv@gmail.com

Online published on 10 February, 2017.

Abstract

For plant regeneration through somatic embryogenesis, immature cotyledonary nodes and embryonic axes explants of soybean cultivars JS 335 were cultured on six different medium fortified with MS salts, B5 vitamins and combinations of growth regulators. Between explants, immature embryonic axes demonstrated better regeneration potential as compared to cotyledonary node. For immature embryonic axes culture, better morphogenic callus induction, embryogenesis, organogenesis and plantlet regeneration was observed on MS with 4B1.86N, MS 10N, MS.1B1N and MS.2B1Nas compared to other media formulations. Cotyledonary nodes exhibited higher callus induction, embryogenesis, organogenesis on media with MS4B1.86N, MS. 1B1N, MS 3B6N and higher plantlet regeneration on media with MS.5B1N.

Keywords

Immature embryonic axes, Immature cotyledonary nodes, Regeneration, Soybean, Somatic embryogenesis