Laboratory of Plant Tissue Culture, Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujrat, India
*Corresponding author's e-mail: abhijeetanandha@ymail.com
Online published on 7 May, 2018.
An efficient method for plant regeneration via callus formation has been developed in pigeonpea using two varieties as BDN-2 and GT-101. Three different explants viz. leaf, hypocotyl and root excised from 15–20 days old in vitro raised seedlings were cultured on basal MS medium. Greenish coloured callus were observed using various media according to explants. More shoots were found in MS + 1.0 mg/l NAA + 4.0 mg/l Adenine and roots on Elongated shoots in MS + 1.0 mg/l IAA media. Rooted plants were transferred in pot for acclimatization.
Callus, In vitro, Pigeonpea, Regeneration
