1National Center for Soybean Improvement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095
2College of Horticulture, Nanjing Agricultural University, Nanjing, 210095
National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, China
*Corresponding author's e-mail: ylzhu@njau.edu.cn
Online published on 5 November, 2018.
Using a quartz sand culture, comparisons were made between the T5 homozygous transgenic lines (HTLs) that overexpress StP5CS (GenBank accessionnumber: JN606861) and their wild-type (WT)host cultivar to examine thedifferences inthe growth and development traits, the concentrations of proline in vegetable soybean [Glycine max (L.) Merrill] under salt stress conditions. Moreover, the relative expression levels of two glutamine synthetase-related genes (GmGS1β1, GmGS1β2), two nodulation-related genes (GmENOD40-1, GmENOD40-2), and one leghemoglobin gene (GmLba) were also measured. The purposeof this research wasto provide a theoretical basis forelucidatingthe mechanisms ofnodulation andnitrogen fixation in the roots of transgenic plants under salt stress conditions. Compared with WT plants, the plant height and seed weight per plant of T5 HTLs significantly increased, and the contents of proline in various tissues of T5 HTLs were also significantly elevated. Quantitative real-time PCR (qRT-PCR) analysis indicated that the expression levels of GmGS1β1, GmGS1β2, GmENOD40-1, GmENOD40-2, and GmLba were significantly increased in T5 HTLs under salt stress conditions. These results indicate that the overexpression of StP5CS in T5 HTLs enhanced growth, nodulation and nitrogen fixation in transgenic vegetable soybean under salt stress conditions.
Nitrogen fixation, Nodulation, Salt Stress, StP5CS Gene, Vegetable soybean
