1ICAR-Indian Institute of Maize Research, New Delhi-110 012, India
Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, Himachal Pradesh, India
*Corresponding author's e-mail and address: indudehal10@gmail.com, Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 004, India
Online published on 27 September, 2019.
Ten morpho-agronomic traits and80 random amplified polymorphicDNA(RAPD) molecular markerswereused tosurvey genetic diversity in 25 chickpea genotypes. Analysis of variance revealed significant variability among different genotypes for morpho-metric traits. The cluster analysis done using morpho-metric traits grouped 25 genotypes into seven and six clusters in Environment I (Env. I) and Environment II (Env. II), respectively. Three genotypes viz., ICCV-96904, HPG-17, ICCV-95503 and L-HR-1 belonging to diverse clusters were identified divergent and may use in heterosis breeding programme. Of 80 random RAPD markers, 25 were found polymorphic. Three major clusters were identified using 25 polymorphic RAPD markers. The genetic similarity coefficient among genotypes ranged from 0.57 to 0.91. The average polymorphic information content (PIC) for 25 RAPD markers ranges from 0.12 to 0.40. D2-statistic, RAPD analysis and study of genotypes performance revealed sufficient genetic diversity among chickpea genotypes which would be useful in future breeding programme.
Cicer arietinum L, Genetic diversity, Morpho-metric traits, RAPD markers
