Hangzhou National Sub-center of Soybean Improvement, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, P.R. China
*Corresponding Author: S.C. Xu, Hangzhou National Sub-center of Soybean Improvement, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences. Hangzhou, Zhejiang, 310021, P.R. China. Email: shengchun2001@163.com
Online published on 29 February, 2020.
Single nucleotide polymorphisms (SNPs) have been proved to be powerful markers in genetic analysis due to their high abundance and polymorphism in plant genomes. The recently developed high-resolution melting (HRM) analysis method provides a novel, quick, and close-tube PCR approach to analyze SNP variations. In present study, 101 HRM-based SNP markers from 20 soybean chromosomes were developed for genotyping vegetable soybean cultivar ‘Zhexian No.9 ’with ‘Williams 82 ’as reference. 33.7% of these markers were polymorphic between ‘Zhexian No.9 ’and ‘Williams 82'. Polymorphic markers were found on 85% (17 of 20) of the soybean chromosomes when comparing ‘Zhexian No.9 ’and ‘Williams 82'. Finally, an array of 101 in-sequence nucleotide letters was generated as the first precise SNP fingerprint of ‘Zhexian No.9'. The described marker-developing methodology could be used in other crops with known genomic information.
HRM, SNP fingerprint, Soybean, Zhexian No.9
