1ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, Gujarat-387 310, India
2ICAR-Indian Institute of Horticultural Research, Bengaluru-560089, Karnataka, India
3ICAR-Central Plantation Crops Reserch Institute, Kasargod-671124, Kerala, India
4Horticultural Research Station, Lam Farm, Guntur, Dr. YSHU, Venkataramannagudem, Andhra Pradesh, India
5PJS Telangana State Agricultural University, Hyderabad-500 030, Telangana, India
*Corresponding Author: A. Chinapolaiah, ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, Gujarat-387 310, India, Email: acpolireddy.hortico@gmail.com
Online published on 29 February, 2020.
Velvet bean is an important medicinal legume, its seeds are prominent source of L-Dopa. The present investigation on genetic diversity assessment of 58 germplasm of velvet bean by using 11 ISSR markers. Out of 63 amplified products 59 were showed polymorphism and 4 were monomorphic with an average of 5.7 bands amplified per primer. According to band statistics and efficiency parameters showed the primers UBC 827, UBC 834 and UBC 836 were more efficient. The highest genetic similarity values (0.90) were observed between IIHR MP 102 and IIHR MP 74-3. In dendrogram germplasm grouped into two major clusters at 63 per cent similarity. Among the germplasm, IIHR Selection 4, IIHR Selection 10, IIHR MP 9, IC 33243 and IIHR MP 7 were found to be distinctly divergent, can be used in the further breeding programme.
Genetic diversity, Germplasm, ISSR markers, L-Dopa, Mucuna pruriens, Polymorphism
