Genetic diversity analysis of advanced breeding lines derived from interspecific and intervarietal crosses of black gram based on morphological and molecular markers

The main objective of this study was to assess the genetic diversity of 34 genotypes comprising of 25 advanced derivatives of interspecific cross between V. mungo x V. umbellata, five intervarietal crosses along with four checks (including blackgram parents) using 11 morphological traits and molecular markers (RAPD and ISSR) and for their resistance towards Cercospora, anthracnose and MYMV. The lines Palampur-93 x BRS-1 (236-A-L-1-4), HPBU-35 and HPBU-111 were found statistically at par to the best check Him Mash-1 for seed yield. Correlation studies revealed that the selection should be based on large seeds, more number of pods per plant and more number of seeds per pod indicating the importance of these traits in yield determination. The cluster analysis on molecular basis grouped the blackgram parents and advance lines differently than at morphological level which revealed genetic variation among genotypes and also confirmed that rigorous selection had been made for blackgram types in segregating generations rather than ricebean types as most of the advance derivatives of V. mungo x V. umbellata were found to be concentrated near blackgram parents. The lines PDU-1 x PRR-1 (62-3-L-10-1) and Palampur-93 x PRR-1 (258-1L-2-5) were found resistant to both Cercospora and anthracnose while, Palampur-93 x BRS-1 (236-A-L-3-2) for MYMV. The information generated from this study would be helpful in characterizing the advanced derived lines and parents in the selection and utilization of diverse genotypes to enhance variability and productivity along with resistance breeding of V. mungo.