Legume Research - An International Journal
Web of Science
  • Year: 2021
  • Volume: 44
  • Issue: 6

Reference genes for quantitative real-time PCR analysis of gene expression in mung bean under abiotic stress and Cercospora canescens infection

  • Author:
  • X.W. Ke1*, L.H. Yin1*, J. Xu1, W.N. Sun1, X.D. Xu1, Y.X. Guo1, Y.H. Zuo1*
  • Total Page Count: 6
  • Page Number: 646 to 651

1Heilongjiang Bayi Agricultural University, National Coarse Cereals Engineering Research Center, Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control, Daqing163319, China

*Corresponding Author: Y.H. Zuo, Heilongjiang Bayi Agricultural University, National Coarse Cereals Engineering Research Center, Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control, Daqing163319, China, Email: zuoyhu@163.com

*These two authors contributed equally to this work.

Online published on 22 July, 2021.

Abstract

The objective of the study was to identify suitable reference genes that can be used for quantitative real-time PCR (qPCR) analysis in mung bean (Vigna radiata). Therefore, 10 potential reference genes were selected and the results showed that ubiquitin-conjugating enzyme was suitable as reference under drought and pathogen infection stress; elongation factor 1 was the most stable gene under waterlogging; and actin performed the best under saline stress. These selected reference genes were further confirmed by analysis of the expression profiles of catalase and peroxidase under waterlogging. Our results will contribute to the improvement of the accuracy of gene expression evaluation in mung bean.

Keywords

Drought, Fungal infection, Qpcr, Saline, Vigna radiata, Waterlogging