1Soybean Breeder, Agricultural Research Station, Kasbe Digraj- 416 305, Sangli, Maharashtra, India
State Level Biotechnology Centre, Mahatma Phule Krishi Vidyapeeth, Rahuri-413 722, Maharashtra, India
*Corresponding Author: M.P. Deshmukh, Soybean Breeder, Agricultural Research Station, Kasbe Digraj-416 305, Sangli, Maharashtra, India. Email: drmpdeshmukh@gmail.com
Online published on 19 February, 2022.
In soybean yellow seed coat is preferred in the market, however, colored ones are currently gaining attention because of their medicinal and nutritive values; besides. Hence it is essential to breed varieties with desired seed coat colour.
Twelve genotypes with six each having yellow and black seed coats were screened with fourteen primers linked to seed coat colour governing loci.
Out of them twelve primers showed polymorphism. Monomorphism was observed with both T loci specific and two of the three R loci specific primers. However I locus specific primers i.e. SM303, SM305 and TR showed polymorphism shared by their seed coat color. SM303 amplified a 180 bp sized band in yellow seed coated genotypes and a 130 bp band in black seed coated genotypes. SM305 amplified dual bands with a 200bp band being monomorphic and an additional band (192–216 bp range) present in only yellow seed coated genotypes, of which a 208 bp band was shared by four yellow seed coated genotypes. Cold induced seed coat discoloration specific TR primer generated bands of different size ranges in yellow seed coated (336–344 bp) and black seed coated genotypes (300–320), of which a 340 bp band was shared by four yellow seed coated genotypes.
Anthocyanin, Inhibitor locus, Markers, Soybean, Seed coat
