Legume Research - An International Journal
Web of Science
  • Year: 2022
  • Volume: 45
  • Issue: 7

Identification of Highly Polymorphic Molecular Markers and Potential Genotypes for Harnessing Chickpea Breeding Strategies

  • Author:
  • Ashwani Kumar1,2,3, Ashwani Yadav1,3, Renu Yadav1,4, J.P. Misra1,3, R.S. Yadav2, H.D. Upadhyaya5, Rajendra Kumar1,3,6,
  • Total Page Count: 11
  • Page Number: 804 to 814

1Department of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110, Uttar Pardesh, India

2Department of Botany, DAV College, Muzaffarnagar-251 001, Uttar Pardesh, India

3UP Council of Agricultural Research, Lucknow-226 010, Uttar Pardesh, India

4Amity University, Noida-201 313, Uttar Pardesh, India

5International Crops Research Institute for the Semi-Arid Tropics, Patancheru-502 324, Hyderabad, Telangana, India

6Division of Genetics, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi-110 012, India

*Corresponding Author: Rajendra Kumar, Department of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110, Uttar Pardesh, India, Email: rajendrak64@yahoo.co.in

Online Published on 02 August, 2022.

Abstract

STMS markers and morphological traits were used to investigate the genetic relationship and allelic diversity in chickpea. In this study, we focused on the selection and more efficient utilization of core germplasm in breeding programs for chickpea crop improvement using STMS and quantitative/morphological traits.

Seeds of elite accessions of chickpea were obtained from ICRISAT, Patancheru, Andhra Pradesh, India. 50 STMS markers and 11 quantitative traits were used for exploring the genetic variability and relationship in 35 chickpea accessions.

A total of 97 alleles were produced out of the 32 polymorphic STMS loci with an average of 3.03 alleles per locus ranging between 2–6 alleles per primer. The PIC value ranged from 0.029 to 0.768 with an average of 0.502. PIC value showed a highly positive correlation (r = 0.718) with number of alleles at the STMS loci. In both molecular and morphological markers/traits-based clustering, out of 35 chickpea accessions only one accession ICC-13892 was isolated at the end of clustering. The results indicated that highly polymorphic microsatellite markers NCPGR 68, NCPGR 50, NCPGR 81, NCPGR 48 and NCPGR 77 along with the accessions ICC-13892 having distant associations with ICC-13816, ICC-15697, ICC-15610, ICC-15868, ICC-15888, ICC-15996 with novel findings should be useful resources for strategies of allele mining, association genetics, mapping and cloning of gene(s) and in applied breeding to broaden the genetic base of chickpea.

Keywords

Allelic diversity, Cicer arietinum L, DNA fingerprinting, Genetic diversity, Polymorphic information content, STMS