1Department of Biology, Faculty of Sciences, Zonguldak Bulent Ecevit University, Zonguldak, Turkey
2Department of Pharmaceutical Botanical, Faculty of Pharmacy, Zonguldak Bulent Ecevit University, Zonguldak, Turkey
3Institute of Science, Zonguldak Bulent Ecevit University, Zonguldak, Turkey
4Department of Biology, Faculty of Sciences, Ankara University, Ankara, Turkey
*Corresponding Author: H. Colgecen, Department of Biology, Faculty of Sciences, Zonguldak Bulent Ecevit University, Zonguldak, Turkey, Email: haticecolgecen@gmail.com
Online published on 13 February, 2023.
Bituminaria bituminosa is a long-day plant. The plant has a high pastoral value and palatability. It is grazed by cattle, goats and sheep. Leaves and stems contain four furanocoumarins’ psoralen, angelicin, xanthotoxin and bergapten. The seed is covered by the fruit. Germination was not observed in beaked fruits. A cut with a scalpel can increase the germination rate. According to the literature there are not enough studies about the culture media and convenient conditions.
In our study, we tried six medium for callus production with different hormone concentrations including 2,4-D, BAP and kinetin. In this study, a protocol for callus induction and analysis of isoflavonoids and terpenes of Bituminaria bituminosa has been developed.
Maximum callus induction frequency was observed on Murashige and Skoog (MS) medium supplemented with 0.75 mg/L 2,4- D + 2.5 mg/L kinetin in young primary leaves. 0.75 mg/L 2,4-D+2.5 mg/L kinetin medium is more successful in the production ofses of flavonoids that can be produced in vitro and is more successful in producing phenols. In terpene production, it is seen that 1.25 mg/L 2,4-D+3.5 mg/L BAP, which is the best callus producing medium containing BAP, is the most successful medium among all tested mediums.
Bituminaria bituminosa, Callus, Isoflavonoids, Terpens
