1Gandhi Krishi Vigynana Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India
2University of Agricultural Sciences, Dharwad-580 005, Karnataka, India
3Jagannath University, Jaipur-302 022, Rajasthan, India
*Corresponding Author: Sunil Subramanya, Gandhi Krishi Vigynana Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India, Email: sunilae929@gmail.com
Online Published on 07 November, 2023.
Fusarium wilt is one of the widely distributed biotic stress of chickpea limiting its productivity worldwide. The major problem limiting the resistance breeding is screening of germplasm and breeding lines for disease resistance. To address such problems, identification of molecular marker closely linked to resistance locus is an effective strategy.
The RAPD marker A07C417 closely linked H2 locus of Fusarium wilt resistance locus has been converted into SCAR and the loss of initial polymorphism was recaptured by alignment of consensus sequences from SCAR amplified locus for the identification of common motifs. Further, the linkage association of this marker with Fusarium wilt resistance locus has been reaffirmed using Fusarium wilt response of RILs phenotyped for wilt reaction using wilt sick pots and also wilt sick plots.
Identification of single nucleotide polymorphism at the consensus SCAR locus between susceptible and resistant cultivar, enabled development of CAPS marker through suitable restriction enzyme Aci I making it an effective codominant marker system for resistance deployment via marker assisted selection.
CAPS, Chickpea, Fusarium, Linkage, SNP
