Legume Research
Web of Science
  • Year: 2023
  • Volume: 46
  • Issue: 11

Prioritization of Microsatellite Markers Linked with Drought Tolerance Associated Traits in Chickpea (Cicer arietinum L.)

  • Author:
  • Prakash N. Tiwari1, Sharad Tiwari1,*, Swapnil Sapre1, Anita Babbar2, Niraj Tripathi3, Sushma Tiwari4, Manoj Kumar Tripathi4,**
  • Total Page Count: 9
  • Page Number: 1422 to 1430

1Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482 004, Madhya Pradesh, India

2Department of Genetics and Plant Breeding, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482 004, Madhya Pradesh, India

3Directorate of Research Services, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482 004, Madhya Pradesh, India

4Department of Plant Molecular Biology and Biotechnology, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior-474 002, Madhya Pradesh, India

*Corresponding Author: Sharad Tiwari, Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482 004, Madhya Pradesh, India, Email: stiwari@jnkvv.org

**Corresponding Author: Manoj Kumar Tripathi, Department of Plant Molecular Biology and Biotechnology, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior-474 002, Madhya Pradesh, India, Email: drmanojtripathi64@gmail.com

Online Published on 01 December, 2023.

Abstract

Being a vital source of high-quality dietary protein, chickpea is an unavoidable legume. The present investigation was performed to study the applicability of the microsatellite markers linked with drought tolerance in Indian chickpea genotypes collected from different genetic background.

In Rabi 2021-22, forty chickpea genotypes including national check for drought tolerance, elite cultivars, released varieties and advanced breeding lines were screened employing forty microsatellite markers linked with drought tolerance associated traits.

Among forty drought tolerance related microsatellite markers, twenty-six were found to be polymorphic and produced a total of 66 alleles, with a mean of 2.5 alleles per locus. Model-based population structure analysis clearly distinguished the drought tolerant genotypes including ICC4958, JG74, JAKI9218, JG16, JG6, JG14 and JG11. The principle coordinate analysis (PCoA) and analysis of molecular variance (AMOVA) further confirmed these results. Findings of the present investigation would have a greater potential for further utilization in breeding of drought specific chickpea cultivar(s).

Keywords

Chickpea, Drought tolerance, Microsatellite markers, Polymorphic information content, Population structure