1Department of Plant Pathology, College of Agriculture, RVS Agricultural University, Gwalior-474 002, Madhya Pradesh, India
2Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior-474 002, Madhya Pradesh, India
3Directorate of Research Services, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482 004, Madhya Pradesh, India
*Corresponding Author: M.K. Tripathi, Department of Plant Molecular Biology and Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior-474 002, Madhya Pradesh, India, Email: drmanojtripathi64@gmail.com
Online Published on 21 March, 2023.
The prevailing methods for the genomic DNA extraction of Sclerotium rolfsii from its mycelium mat is often time consuming and yields poor quality and quantity of genomic DNA owing to presence of the outrageous magnitude of mucilage and polysaccharides.
A new fast track method for DNA isolation from the resting structure (sclerotia) of S. rolfsii by modifying CTAB method deprived of supplementation of proteinase K has been standardized.
The protocol produced 500ng DNA from sclerotia with purity vacillating from 1.7 to 1.9 as confirmed by A260/A280 and A260/A230 spectrophotometric documentation. The DNA extracted from sclerotia commissioning protocol was efficaciously used for the further downstream reactions/process like PCR-RAPD, PCR-ISSR and ITS amplification of rDNA-ITS region.
DNA extraction, ISSRITS, PCR, RAPD, Sclerotia, Sclerotium rolfsii
