Legume Research - An International Journal
Web of Science
  • Year: 2023
  • Volume: 46
  • Issue: 3

Association of Seed Amylose Content with Agronomic Traits and GBSS1a Haplotypes in Common Bean Landraces

  • Author:
  • Sookyeong Lee1, Do Yoon Hyun1, Kyung Jun Lee2, Seungbum Lee1, Gyu-Taek Cho1,*, Hee-Jong Koh3,**
  • Total Page Count: 7
  • Page Number: 281 to 287

1National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju54874, Republic of Korea.

2Honam National Institute of Biological Resources, Mokpo58762, Republic of Korea.

3Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul08826, Republic of Korea.

*Corresponding Author: Gyu-Taek Cho, National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju54874, Republic of Korea, Email: gtcho@korea.kr

**Corresponding Author: Hee-Jong Koh, Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul08826, Republic of Korea, Email: heejkoh@snu.ac.kr

Online Published on 06 April, 2023.

Abstract

Common bean (Phaseolus vulgaris L.) is a valuable source of starch, proteins and minerals in the human diet. Starch, a major component of bean seeds, is composed of amylose and amylopectin and the content of amylose is important for affecting the quality of bean-derived food products. However, limited information is available on amylose assessment in bean.

In this study, for a total of 300 bean landraces, we evaluated the seed amylose content and characterized twenty agronomic traits and analyzed the granule-bound starch synthase 1a (GBSS1a) gene haplotypes, which are likely to be associated with amylose content. Also, the correlation of amylose content with agronomic traits and GBSS1a gene haplotypes were determined.

Bean landraces showed diverse phenotypes, highly variable amylose content (39.95-63.57%) and three GBSS1a haplotypes (YP, YQ and NP). Correlation analysis revealed that seed size and seed weight were significantly correlated with amylose content and NP-type beans contained more amylose than YQ-type beans. Although these correlations could not fully explain the tremendous amount of variation in seed amylose content in bean germplasm, information on starch composition-related GBSS1a haplotypes and agronomic traits would facilitate genetic analyses and breeding research targeted toward improving the composition of bean starch.

Keywords

Agronomic trait, Amylose, Common bean, GBSS1a gene, Haplotype, Landrace, Starch