1Division of Crop Improvement, ICAR-Central Research Institute for Jute and Allied Fibers, Barrackpore-700 120, Kolkata, West Bengal, India
2Division of Genetics, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi-110 012, India
*Corresponding Author: Jitendra Kumar Meena, Division of Crop Improvement, ICAR-Central Research Institute For Jute and Allied Fibers, Barrackpore-700 120, Kolkata, West Bengal, India, Email: jkdhanawat01@gmail.com
Online published on 12 July, 2023.
Vascular wilt caused by Fusarium oxysporum f.sp. lentis Vasu. and Srini. is a serious disease of lentil (Lens culinaris Medikus), causes severe yield losses worldwide. For effective disease resistance breeding the inheritance and mapping of wilt resistance gene (s) is necessary. Therefore, the present investigation was focused on study the mode of inheritance and tag/map gene (s) for fusarium wilt resistance in lentil.
Bulked segregant analysis (BSA) approach was used to identify markers that were tightly linked to Fusarium wilt resistance gene. The inheritance and mapping of wilt-resistance gene (s) in lentil was investigated in F2 and F2:3 populations derived from L9-12×ILL10965 cross, whereas L9-12 and ILL10965 were susceptible and resistant parents, respectively.
More than two hundreds SSRs markers were surveyed for the parental polymorphism, of which twenty nine were found polymorphic. These polymorphic SSRs were used for the bulked-segregant analysis (BSA) using both parents and its respective resistant and susceptible bulks, and three SSRs viz. PBALC233, PBALC1409 and PBALC203 could distinguish the respective bulks. Linkage analysis showed two SSR markers, PBALC203 and PBALC1409 flanking the wilt resistance gene at 8.2 cM and 9.4 cM distance, respectively. Further, PBLAC233 was also found present on the same linkage group at a distance of 10.2 cM from PBLAC1409.
Fusarium oxysporum, Lens culinaris, Linkage, Mapping
