1College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, China
*Corresponding Author: Fengling Shi, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, China, Email: nmczysfl@126.com
Online published on 31 July, 2023.
To accelerate the establishment of a genetic improvement system for Onobrychis viciaefolia Scop. by cultivating haploid plants and producing pure parental material.
We endeavored to set up the Onobrychis viciaefolia Scop. anther culture system, examine the suitable anther pre-treatment method and the factors influencing medium type and hormone ratio during anther regeneration.
The regenerative capacity of intact anthers was preserved by 48 h low temperature (4°C) medium treatment. Or the broken anthers were obtained when the regenerated grass was budding and preserved in the medium after 48 hours of low temperature treatment; callus induction was based on MS and the callus rate was 18.33% when 1.0 mg/L 2,4-D + 0.75 mg/L 6-BA + 0.8 mg/L NAA + 2.0 mg/L KT was added. With N6 as the basic medium, the addition of 2.0 mg/L 2,4-D + 0.75 mg/L 6-BA + 0.4 mg/L NAA + 0.5 mg/ L KT resulted in a healing rate of 16.19%; the addition of 0.5 mg/L 6-BA + 1.0 mg/L KT to the MS medium resulted in a differentiation rate of 68%; during the construction of the anther histoponic system, browning and waterlogging of the healing tissues and vitrification of the seedlings hindered the formation of regenerated plants.
Anther, Callus rate, Differentiation rate, Onobrychis viciaefolia Scop ‘Mengnong’
