1Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India
2Sugarcane Research Station, Tamil Nadu Agricultural University, Melalathur, Vellore-635 806, Tamil Nadu, India
3Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400 085, Maharashtra, India
4Department of Crop Physiology, Directorate of Crop Management, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India
*Corresponding Author: R. Sudhagar, Sugarcane Research Station, Tamil Nadu Agricultural University, Melalathur, Vellore-635 806, Tamil Nadu, India, Email: sudhagar.r@tnau.ac.in
Online published on 5 September, 2023.
Two horsegram varieties viz., PAIYUR 2 and CRIDA1-18R were mutated with gamma rays (G), electron beam (EB), G+EB and G+EMS (ethyl methane sulphonate) to determine the mutagenic potency in breeding programme.
Uniform seeds treated with different mutagenic doses were raised in randomized block design which constituted M1 generation. Each plant was harvested individually and forwarded to M2 generation following plant to progeny row method.
A dose dependant decline was observed for seed germination, plant survival, root length, shoot length, plant height, pollen fertility and seed fertility in M1 population. Wide spectrum of chlorophyll mutants was induced in M2 generation with maximum frequency at EB followed by combination treatments (G+EMS and G+EB) in both varieties. The mutagenic effectiveness ranged from 0.14 per cent to 1.45 per cent in PAIYUR 2 and 0.15 per cent to 1.71 per cent in CRIDA1-18R. The high mutation rate for effectiveness was exhibited by G and G+EB. With reference to sterility, EB was found to be efficient mutagen in both varieties whereas varied efficiency was noted for lethality (EB - PAIYUR 2; G+EMS - CRIDA1-18R) and injury (G - PAIYUR 2; G+EMS - CRIDA1-18R).
Chlorophyll mutants, Effectiveness, Efficiency, Horsegram, Induced mutation
