1Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1 Nishi Gakuen-kibanadai, Miyazaki, 889-2192, Japan
2Faculty of Agriculture, University of Miyazaki, 1-1 Nishi Gakuenkibanadai, Miyazaki, 889-2192, Japan
3Faculty of Regional Innovation, University of Miyazaki, 1-1 Nishi Gakuen-kibanadai, Miyazaki, 889-2192, Japan
4University of Miyazaki, 1-1 Nishi Gakuen-kibanadai, Miyazaki, 8892192, Japan
5Frontier Science Research Center, University of Miyazaki, 1-1 Nishi Gakuen-kibanadai, Miyazaki, 889-2192, Japan
*Corresponding Author: Takahiro Gondo, Frontier Science Research Center, University of Miyazaki, 1-1 Nishi Gakuenkibanadai, Miyazaki, 889-2192, Japan, Email: gondo@cc.miyazaki-u.ac.jp
Online Published on 08 July, 2025.
Identifying biomass-related genes to meet the increasing demand for food supply is important approach for agricultural and scientific efforts. In this study, we employed the Full-length cDNA Over-eXpressing gene hunting system (FOX hunting system) to identify biomass-related genes through ectopic expression of Arabidopsis thaliana full-length cDNA in bird’s-foot trefoil (Lotus corniculatus).
A total of 66 FOX superroot lines (FSLs) from Lotus corniculatus cv. Viking were transferred in vermiculite after two weeks in half-strength Murashige and Skoog (MS) medium. These FSLs were then grown in a growth chamber for four weeks, during which their plant length and root length were measured. High biomass lines were selected from this group. FSL #83 and #121 were then grown for an additional four months in a greenhouse until they reached maturity, at which point they were evaluated for their agronomic traits.
Out of the 66 analyzed FSLs, 15 displayed superior growth characteristics in plant length and total root length. Arabidopsis cDNA, categorized into genes related to various functions such as membrane transport, photosystems, phytohormone synthesis, amino acid synthesis and unknown genes, was introduced into these lines. Two lines were grown to maturity in the greenhouse and their agronomic traits were analyzed in detail. FSL #83 expressed brassinosteroid biosynthetic pathway gene (CPD) led to significant differences in internode length, number of stems, number of flowers and above-ground dry matter weight compared to non-transgenic Super-growing roots (SR). On the other hand, FSL #121 expressed Asparaginyl-tRNA synthetase (SYNC1) gene exhibited increases in plant length and the number of stem nodes.
Biomass-related genes, CPD, FOX gene-hunting system, Lotus corniculatus, SYNC1
