Legume Research
Web of Science
  • Year: 2025
  • Volume: 48
  • Issue: 11

Identification and Molecular Characterization of Thrips palmi (Karny 1925): A Potential GBNV Vector in Blackgram using ITS2 Marker

  • Author:
  • Rajasekhar Lella1,*, Tirumalasetti Madhumati2, D.V. Sairam Kumar2, V. Prasanna Kumari3, V. Roja4
  • Total Page Count: 11
  • Page Number: 1835 to 1845

1Department of Agriculture, Government of Andhra Pradesh, India

2Department of Entomology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla-522 101, Andhra Pradesh, India

3Department of Plant Pathology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla-522 101, Andhra Pradesh, India

4Department of Biotechnology, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur-522 034, Andhra Pradesh, India

*Corresponding Author: Rajasekhar Lella, Department of Agriculture, Government of Andhra Pradesh, India, Email: rajagrico724@gmail.com

Online Published on 10 February, 2026.

Abstract

Identification and characterization of T. palmi a potential vector of GBNV in blackgram.

Pictorial taxonomic key-based morphological identification of T. palmi and molecular characterization through DNA sequencing using species specific marker ITS2 for accurate confirmation of the species.

The present paper reports the host record of T. palmi on blackgram and its identification, molecular characterization through DNA barcodes from Andhra Pradesh. This study contributed 07 (seven) novel gene sequences to NCBI database and also revealed the existence of low genetic polymorphism among the ITS2 sequences of T. palmi. Species specific ITS2 primer was found promising. Six more genera of thrips were also identified viz. Megalurothrips usitatus (Bagnall), Scirtothrips dorsalis (Hood), M. typicus (Bagnall), Ayyaria chaetophora (Karny), Phibalothrips peringueyi (Faure) and some Tubuliferan thrips. T. palmi found in all GBNV hotspots of blackgram across Andhra Pradesh.

Keywords

DNA barcodes, Genetic polymorphism, ITS2, T. palmi