1Department of Plant Breeding and Genetics, Assam Agricultural University, Jorhat-785 013, Assam, India
2Department of Crop Physiology, Assam Agricultural University, Jorhat-785 013, Assam, India
3Department of Horticulture, Assam Agricultural University, Jorhat-785 013, Assam, India
*Corresponding Author: Thalari Vasanthrao, Department of Plant Breeding and Genetics, Assam Agricultural University, Jorhat-785 013, Assam, India, Email: vasanthmosestalari333@gmail.com
Online published on 10 March, 2026.
The investigation on “Mutation induction in garden pea (Pisum sativum L.)” was carried out to study the mutagenic effects of ethyl methane sulphonate (EMS) on quantitative traits in M1 generation and determine the effectiveness and efficiency of different doses of EMS.
The two varieties of garden peas, Kashi Nandini and Kashi Uday, were subjected to mutagenic treatments with the EMS at 0.05, 0.10, 0.15, 0.20, 0.30 and 0.40%. The M1 generation was raised in RBD with two replications during Rabi 2019-20. Plants from three concentrations, 0.10, 0.15 and 0.20% EMS, were evaluated in M1 plant progenies (M2) during Rabi 2020-21.
Two varieties of garden pea exposed to EMS showed varied responses to different concentrations. The inhibitory effects were apparent in germination, survival and pollen sterility in both varieties. The mean performances in the M1 generation showed differential responses to different concentrations of EMS. Mutagenic effectiveness decreased with increased EMS concentrations. Mutagenic efficiency based on pollen sterility decreased and based on lethality increased with increased EMS concentrations. In subsequent generations of mutagenized populations, the plants with altered phenotypes might produce mutants with higher yields.
Chlorophyll mutants, EMS, Garden pea, LD50, Mutagenic effectiveness, Mutagenic efficiency, Pollen sterility
