Legume Research
Web of Science
  • Year: 2025
  • Volume: 48
  • Issue: spl

Assessment of Molecular Diversity and Mapping of Bruchid Tolerance Loci in Green Gram [Vigna radiata (L.) Wilczek]

  • Author:
  • Y. Soujanya1, B. Sukrutha1, Srividhya Akkareddy2,*, Devaki Kayam1, V.L.N. Reddy1, L. Prasanthi2
  • Total Page Count: 9
  • Page Number: 62 to 70

1Department of Genetics and Plant Breeding, S.V. Agricultural College, Acharya NG Ranga Agricultural University, Tirupati-517 502, Andhra Pradesh, India

2Department of Plant Breeding, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya NG Ranga Agricultural University, Tirupati-517 502, Andhra Pradesh, India

*Corresponding Author: Srividhya Akkareddy, Department of Plant Breeding, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya NG Ranga Agricultural University, Tirupati-517 502, Andhra Pradesh, India, Email: a.srividhyaphd@angrau.ac.in

Online published on 6 February, 2026.

Abstract

Bruchid poses threat to the greengram production and development of tolerant varieties avoids heavy losses.

A total of 47 diverse genotypes were screened for bruchid (Callasobruchus chinensis) tolerance and available molecular variation at reported bruchid tolerant loci there by the markers associated with tolerance.

ANOVA of pod and seed characters along with bruchid infestation (%) revealed significant variation. Bruchid incidence at 40 days after treatment (DAT) was ranged from 19.67% (OBGG-57) to 99.33% (NRI-SAKTHI-POLISH) with 59.38% mean. When tested for long term tolerance, at 150 DAT, OBGG-57 alone remained as resistant with 28.67% seed infestation. Hence, OBGG-57 can be used as bruchid resistant genotype directly for release/as donor in breeding programmes. Molecular screening targeting reported bruchid tolerant loci revealed high informativeness for DMBSSR125 marker with PIC of 0.90. Association analysis revealed the linkage of DMBSSR125 (EST-SSR) to bruchid tolerance with p-value of 0.0378. BLAST analysis of the marker sequence against greengram genome aligns to two Metallothionein (MTs) proteins (vigra. Vradi09g07610, Vradi0111s00070). MTs are small cysteinerich proteins. A cysteine-rich protein, ‘VrCRP’ has been reported for wild greengram (TC1966) tolerance towards Callasobruchus chinensis. Hence, DMBSSR125 that corresponds to a cystine-rich protein can be further validated through mapping/introgression approaches to confirm association and to use in bruchid resistant breeding programmes.

Keywords

Association study, Bruchid tolerance, Diversity, Greengram, Pod and seed traits