Legume Research
Web of Science
  • Year: 2026
  • Volume: 49
  • Issue: 1

Morpho-pathogenic and Molecular Variability of Chickpea Collar Rot in Bundelkhand Region of Uttar Pradesh, India

  • Author:
  • Arvind Kumar1*, Virendra Kumar Singh1, Vivek Singh2, Ashutosh Rai3, Deo Kumar4, Rishi Nath Pandey1, Girijesh Kumar Jaisval5, Himanshu Kumar Gupta6, Ali Khan1
  • Total Page Count: 8
  • Page Number: 152 to 159

1Department of Plant Pathology, College of Agriculture, Banda University of Agriculture and Technology, Banda-210 001, Uttar Pradesh, India.

2Department of Plant Pathology, College of Agriculture, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224 229, Uttar Pradesh, India.

3Division of Vegetable Production, Indian Institute of Vgetable Research, Jakhini, Varanasi-221 305, Uttar Pradesh, India.

4Department of Soil Science and Agricultural Chemistry, College of Agriculture, Banda University of Agriculture and Technology, Banda-210 001, Uttar Pradesh, India.

5Department of Plant Pathology, Krishi Vigyan Kendra, Balrampur, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224 229, Uttar Pradesh, India.

6Department of Plant Pathology, Post Graduate College, Ghazipur, Veer Bahadur Singh Purvanchal University, Jaunpur-222 001, Uttar Pradesh, India.

*Corresponding Author: Arvind Kumar, Department of Plant Pathology, College of Agriculture, Banda University of Agriculture and Technology, Banda-210 001, Uttar Pradesh, India. Email: arvindkumarak638419@gmail.com

Abstract

Collar rot of chickpea infected by Sclerotium rolfsii is considered one of the major biotic factors for low productivity in chickpea. The present study was conducted to determine twenty nine isolates of collar rot were characterized for their morphological, pathogenic and molecular variability.

Twenty nine collar rot infected chickpea plant were collected from different places of Bundelkhand region of Uttar Pradesh. Pathogen was isolated from collar rot infected sample using PDA media followed by morphological, pathogenic and molecular variability studies from all isolates. Type of growth rate, mycelia colour, sclerotia size, sclerotial pattern, test weight of isolates was recorded by observing cultural plate after complete growth of mycelium. Pathogenicity test were done to check the aggressiveness of isolates in three varieties of chickpea. The isolated fungal DNA was amplified with specific primer pair, the quality and quantity of DNA was checked using gel electrophoresis and UV trasnliminator.

A morphological evaluation of isolates showed notable differences in identification characteristics, including colony size and sclerotial properties like count, test weight, dimensions and form on PDA media. Among the all isolates SRC 25 and SRC 28 considered as most virulent causes highest disease incidence in all selected chickpea variety under greenhouse experiments. RAPD analysis revealed distinct banding patterns, with PIC values between 0.59 and 0.89. The highest genetic similarity was observed between SRC-11 and SRC-29.

Keywords

Chickpea, Collar rot, Molecular variability, Morphology, Pathogenicity