Legume Research
Web of Science
  • Year: 2026
  • Volume: 49
  • Issue: 3

Impact of Seed-borne Mycoflora on Seed Quality Parameters in Greengram (Vigna radiata L.)

  • Author:
  • D. Pooja1, C.S. Shantharaja2*, V.K. Deshapande3, Kiran Dasanal3, Dhruv Bhagvatkar4, H.B. Dinesh5, Gurupada Balol6, Gayathri Khandappa Ravaloji1
  • Total Page Count: 10
  • Page Number: 504 to 513

1Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India.

2ICAR-National Institute of Seed Science and Technology, Regional Station, Gandhi Krishi Vignana Kendra, Bengaluru-560 065, Karnataka, India.

3Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Dharwad-580 005, Karnataka, India.

4Department of Seed Science and Technology, ICAR-Indian Agriculture Research Institute, New Delhi-110 012, India.

5Keladi Shivappa Nayaka University of Agricultural and Horticultural Sciences, Iruvakki, Shivamogga-577 417, Karnataka, India.

6All India Coordinated Research Project on Groundnut, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad-580 005, Karnataka, India.

Corresponding Author: C.S. Shantharaja, ICAR-National Institute of Seed Science and Technology, Regional Station, Gandhi Krishi Vignana Kendra, Bengaluru-560 065, Karnataka, India. Email: shantharajacs@gmail.com

Abstract

Greengram (Vigna radiata) is an important pulse crop widely cultivated in tropical and subtropical regions for its nutritional and economic value. However, seed-borne fungal pathogens are a major constraint to its productivity, viability and seed quality. These pathogens infect seeds during various developmental stages, leading to reduced germination rates, lower seed vigor and diminished storage potential. The present study aimed to assess seed-borne fungal infections in greengram genotypes using the agar plate method and to evaluate the efficacy of selected chemical fungicides and biological agents under pot culture conditions.

A total of twenty-four greengram genotypes were screened for seed-borne fungal infections using the agar plate method. The incidence of infection was recorded and fungal species were isolated and identified based on morphological characteristics. To manage these infections, pot culture experiments were conducted using the most susceptible genotype (DGGV 2). The study evaluated the bio-efficacy of selected chemical fungicides (Sprint and Xelora) and biological (Trichoderma harzianum). Seed germination percentage, vigor index, plant growth and yield parameters were recorded and analyzed to assess the effectiveness of the treatments.

Eight seed-borne fungal species were isolated and identified. The genotype TARM 1–28-1 exhibited the lowest infection rate (4.83%) and the genotype DGGV 2 showed the highest infection rate (12.50%). Seed treatment with Sprint and T. harzianum in susceptible genotype DGGV 2 significantly reduced fungal incidence and enhanced seedling growth and yield performance. Xelora was particularly effective in controlling anthracnose caused by Colletotrichum spp. The study highlights the importance of integrating resistant genotypes with effective seed treatment strategies for managing seed-borne fungal diseases and enhancing greengram productivity.

Keywords

Green gram, Mycoflora, Seed quality, Seed treatment, Seedborne diseases