1Department of Agriculture, Guntur-522 004, Andhra Pradesh, India.
2Department of Entomology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla-522 101, Andhra Pradesh, India.
3Department of Plant Pathology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla-522 101, Andhra Pradesh, India.
4Department of Biotechnology, Regional Agricultural Research Station-Lam, Acharya N.G. Ranga Agricultural University, Bapatla-522 101, Andhra Pradesh, India.
Corresponding Author: Rajasekhar Lella, Department of Agriculture, Guntur-522 004, Andhra Pradesh, India. Email: rajagrico724@gmail.com
Investigate the probable insect vector transmitting the GBNV in blackgram.
GBNV isolate maintained on cowpea for transmission studies under greenhouse conditions. RT PCR has been employed to know the presence of GBNV before and after transmission studies. Various interval periods have been fixed to investigate AAP, IAP and number of larvae required to transmit the virus was also determined.
Among the two species tested for GBNV transmission in blackgram, only T. palmi successfully transmitted GBNV whereas M. usitatus, failed to transmit the virus. Therefore, Thrips palmi was identified as the vector for Groundnut bud necrosis virus in blackgram. A minimum of 2 hours of acquisition access period (48 IAP) and 4 hours of inoculation access period (24 h AAP) is needed for first instar larvae of T. palmi. A minimum of 2 h acquisition access period (with 48 h IAP), 8 h inoculation access period (24 h AAP) was observed in case of second instar larvae and a minimum of 10 larvae required to transmit the disease at 24 AAP and 48 h IAP and 2 larvae required to transmit the disease at 48 AAP and 48 h IAP. No disease transmission was observed by adults.
Acquisition access period, GBNV, Inoculation access period, M. usitatus, T. palmi
