1Department of Genetics and Plant Breeding, College of Agriculture, Gandhi Krishi Vigyana Kendra, UAS, Bangalore-560 065, Karnataka, India.
2AICRP on MULLaRP, Main Agricultural Research Station, UAS, Dharwad-580 005, Karnataka, India.
3Department of Biotechnology, College of Agriculture, Gandhi Krishi Vigyana Kendra, UAS, Bangalore-560 065, Karnataka, India.
4Department of Genetics and Plant Breeding, College of Agriculture, UAHS, Shivamogga-577 412, Karnataka, India.
5Department of Genetics and Plant Breeding, College of Agriculture, Vijayapur, UAS, Dharwad-580 005, Karnataka, India.
6AICRP on Seed (Crops), Seed unit, UAS, Dharwad-580 005, Karnataka, India.
7Department of Plant Pathology, College of Agriculture, Dharwad, UAS, Dharwad-580 005, Karnataka, India.
*Corresponding Author: Malagouda D. Patil, AICRP on MULLaRP, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad-580 005, Karnataka, India. Email: patilmd@uasd.in
Flowering time is an important trait of consideration while developing cultivars with desired crop duration. It is wise to understand the variability for the attribute in the population developed through appropriate parental combination for the desired target trait. For early generation testing and use in marker assisted selection, identifying the molecular markers associated with the trait of interest is crucial to enhance precision and decision making.
An F2 population developed through hybridization of contrasting genotypes for flowering time and maturity viz., IPF 4–9 and Khanapur 10 was evaluated with an aim of isolation of superior transgressive segregants and bulk segregant analysis (BSA) was employed for identifying linked molecular markers for flowering time.
The wide range of flowering times, spanning from 24 to 90 days was noticed across the genotypes, in addition, substantial variability was recorded for plant height (range), number of pods per plant (range), days to first flowering (range) and seed yield per plant (range). Studies on genetic parameters indicated high genetic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV), along with high heritability and genetic advance for number of pods per axil, number of primary branches per plant, hundred seed weight and number of seeds per pod. Different classes of transgressive segregants based on flowering time with varied maturity were identified. BSA for flowering time using early and late bulks revealed two SSR markers, AB23 and AA206, linked to the trait. The research outcome of isolation of superior transgressive segregants and molecular markers linked to flowering time will be immensely useful and form genetic resource in developing high yielding early genotypes suitable under changing climatic vagaries and for early generation testing and marker assisted selection (MAS).
BSA, Diversity, Field pea, Flowering, Transgressive segregation
