1Department of Entomology, Sri Venkateswara Agricultural College, Acharya N.G. Ranga Agricultural University, Tirupati-517 502, Andhra Pradesh, India
2Department of Entomology, Institute of Frontier Technology, Regional Agricultural Research Station, Tirupati-517 502, Andhra Pradesh, India
3Department of Genetics and Plant Breeding, Regional Agricultural Research Station, Lam, Guntur-522 034, Andhra Pradesh, India
*Corresponding Author: Debasree Bhowmik, Department of Entomology, Sri Venkateswara Agricultural College, Acharya N.G. Ranga Agricultural University, Tirupati-517 502, Andhra Pradesh, India, Email: debashree7665@gmail.com
Four different pod borers species viz., Spotted pod borer (Maruca vitrata), Tobacco cutworm (Spodoptera litura), Gram blue butterfly (Euchrysops cnejus) and Banner moth (Eublemma dimidialis) were collected from black gram fields of Tirupati district, Andhra Pradesh.
Molecular characterization of mtCOI sequences of the four black gram pod borer species were deposited in NCBI and accession numbers were allotted, M. vitrata (PP938848), S. litura (PP938758), E. cnejus (PP938841) and E. dimidialis (PP938850) respectively. DNA barcodes were generated and BINs were allotted for four different pod borers and available at BOLD public database.
The phylogenetic analysis of all the pod borers together revealed that the pod borer species were distinctly separated at genus level and were grouped into congeneric clusters. The DNA barcode of E. dimidialis (BOLD:AAB6502) was generated for the first time from India and the DNA barcode of E. cnejus (BOLD:AAD0845) was generated for the first time from Andhra Pradesh, India.
Black gram, Eublemma dimidialis, Euchrysops cnejus, Maruca vitrata, Molecular characterization, Pod borers, Spodoptera litura
