The Separation, Isolation, Purification and Characterisation of IgG from Non-Epileptic and Epileptic Patients

Research work on the separation, isolation, purification and characterisation of IgG from non-epileptic and epileptic patients was carried out to examine the unexplored and overcome neurological disorders (epilepsy) with the help of various sophisticated techniques. The results were summarised as follows: (1) The IgG conc. in blood serum of epileptic patients increased as compared to the non-epileptic persons. (2) The IgG conc. in blood serum of non-epileptic and epileptic patients, studied by UV spectroscopy, showed similar results. The increase in IgG conc. in blood serum of epileptic patients is due to increased level of neuron-specific antibodies in the sera. (3) The molecular characterisation of IgG was studied by disc gel electrophoresis. A single protein band was observed in a normal person's pattern; whereas, the protein profile of IgG in abnormal serum samples (E1, E2 and E3) shows one or two protein bands of both IgG and IgA.