1Assistant Professor Biochemistry, Department of Integrated Biotechnology,
2Post Graduate Dissertation Student, Department of Microbiology, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences (ARIBAS), P.O Box No.61, New Vallabh Vidyanagar, Vitthal Udyognagar-388121, Anand, Gujarat, India.
*Corresponding Author Email id: devjani.chakraborty@yahoo.com
The present study was conducted in blood samples collected from Chicken (Gallus domesticus) during the period of December, 2010, from Anand Poultry, Gujarat, India, for isolation, identification and characterisation of pathogenic bacterial species on basis of their morphology, staining properties, cultural and biochemical properties. Seven different isolates were obtained from the blood. All of them were found to be pathogenic. Antibiotic sensitivity and resistivity of the isolated organisms were also studied. Sodium dodecyl sulfate-polyacrylamide electrophoresis demonstrated the presence of α-haemolysin protein of 33 kDa. Further, 16SrRNA was performed to characterise bacteria, which were further confirmed as streptomycin-, penicillin- and MOX-resistant Staphylococcus aureus.
Bacteria, Chicken's Blood, α-Haemolysin, 16SrRNA Characterisation
