Phytochemical Screening and Quantification of Secondary Metabolite in Pluchea lanceolata at Various Stages of Development Using HPTLC

The present study deals with preliminary phytochemical investigations in in vivo (leaf and stem) and in vitro (callus and micropropagated plantlets) plant parts of Pluchea lanceolata. A TLC (thin layer chromatography) densitometric method for quantification of quercetin using HPTLC (high-performance TLC) was developed. Quantification of quercetin was carried out from the methanolic extract using the solvent system ethyl acetate: methanol (1:1 (v/v)). The Rf value of quercetin was 0.83. The linearity ranges for quercetin were 320–640 ng/spot, with a correlation coefficient (r values) of 0.997. The amount of quercetin in leaves, stem, callus and micropropagated plantlets were 322.88, 233.31, 375.85 and 389.92 µg/ml, respectively.