1Research Scholar, PG Department of Biotechnology, Vinoba Bhave University, Hazaribag-825301, Jharkhand, India
2Head, PG Department of Biotechnology, Vinoba Bhave University, Hazaribag-825301, Jharkhand, India
*Corresponding author email id: baby.rai@gmail.com;
The present research was carried out to identify the effect of sodium azide (NaN3) as an alternative method for mutation induction additionally, to find out the effect of NaN3 treatments on gene expression and morphological growth of chlorophytum borivilianum (also known as Safed musli). The experiments have revealed interesting information regarding influence of NaN3 treatments on genetic stability of Safed musli. In the protein profiling of treated leaves two polypeptides were found up regulated only in water immersed explants as compared to control. One of the polypeptides (43.3 KD) was found up-regulated in explants treated for 1 mM, 2 mM and at lesser extent in, 3 mM and 5 mM while 99.8 KD polypeptide was up regulated in all the treatments concentration of NaN3 namely 1 mM, 2 mM, 3 mM and 5 mM. A total of 19 polypeptide species were clearly resolved with a number of additional unclear bands in the gel analysis of control. The molecular weight of these polypeptides ranged from 14.8 KD to 122.2 KD. Two polypeptides were found up-regulated in pot. One of the bands of 122.2 KD was up- regulated in all the treatments namely 1 mM, 2 mM, 3 mM and 5 mM another was 58.3 KD in 2 mM treatment only.
Safed musli, RAPD marker, Sodium azide, Gene expression, Genetic stability, Protein profiling, Polypeptides
